Research Reference
NAD+ — Reconstitution & Research Guide
NAD+ acts on redox coenzyme and is studied for energy, dna-repair & sirtuin research (active research). Below: what the research covers, how to reconstitute and verify a vial, and reference values from the literature — framed strictly for laboratory research.
Research use only. This page is a research and laboratory-reference resource — mechanism, reconstitution math, and reference values from published research. It provides no human, veterinary, therapeutic, diagnostic, or administration guidance. Compounds sold by Renova Wellness™ are not approved for human or animal consumption.
Quickstart
Category
longevity
Vial sizes
500 / 1000 mg
Reconstitution
500 mg in 3 mL → 166.67 mg/mL
Reference amount
50–100 mg (research literature) · ~30 units/draw
Per-batch COA
HPLC purity + mass-spec identity
Storage
Frozen (lyophilized) · 2–8°C reconstituted
What the research covers
NAD+ acts on Redox coenzyme and is studied for energy, dna-repair & sirtuin research. Research status: active research. The evidence base is largely preclinical and mechanistic; this section summarizes what published work has examined, not outcomes for any product. See the full cited overview in the NAD+ research guide.
Reconstitution & calculator
Reconstitution is arithmetic: concentration = mg ÷ mL. A practical option is 5 mL → 100 mg/mL. The tool below is pre-loaded for NAD+; type a vial and reference amount and it returns the clean-tick water options, U-100 units, and draws per vial.
Nova — Renova’s research assistant
Guided reconstitution · ⚡ Powered by Claude
- 1Draw 3 mL of bacteriostatic water (the volume set in the calculator above) and add it slowly down the inside wall of the vial.
- 2Swirl gently until fully dissolved — do not shake. The reconstituted solution should be clear.
- 3Label the vial with the date.
- 4Refrigerate at 2–8°C, protected from light, and use within the handling window below.
Supply reference
At a 50 mg reference amount, a 500 mg vial yields about 10 draws — roughly 3.3 weeks at 3 draws/week. Adjust the amount and cadence in the calculator to plan your own research schedule.
Shop NAD+ →Storage & handling
- • Lyophilized: keep frozen, protected from light and moisture.
- • Reconstituted: refrigerate at 2–8°C, protected from light.
- • Avoid freeze–thaw of the reconstituted solution.
- • Allow a frozen vial to reach room temperature before opening. Read the storage guide.
Verify before you trust it
Every NAD+ lot is third-party tested and ships with its own per-batch Certificate of Analysis — HPLC purity and mass-spectrometry identity, tied to the lot number on your vial. Don’t take our word for it: confirm the exact lot yourself.
NAD+ FAQ
How do I reconstitute NAD+?+
NAD+ ships lyophilized and is reconstituted with bacteriostatic water. Divide the mg in the vial by the mL of water for the concentration: a 500 mg vial in 3 mL is 166.67 mg/mL. Use the calculator on this page for other volumes. This is laboratory math, not a protocol.
What are the reference amounts for NAD+?+
In the research literature NAD+ is commonly referenced around 50–100 mg (research literature). At a 500 mg vial in 3 mL that's about 30 units on a U-100 syringe for a 50 mg draw. These are reference values from published research — not a dose, recommendation, or instruction.
How is NAD+ verified for purity?+
Every Renova Wellness lot ships with its own per-batch Certificate of Analysis — identity by mass spectrometry and purity by HPLC, tied to the lot number on the vial. You can confirm any lot yourself on the verification page before it leaves the bench.
How should NAD+ be stored?+
Lyophilized vials are kept frozen, protected from light and moisture. Once reconstituted with bacteriostatic water, the solution is refrigerated at 2–8°C, protected from light, and avoided from freeze–thaw cycles. General laboratory-handling reference only.
Research references
Primary literature for NAD+. Provided for scientific context only — not medical or dosing guidance.
- Covarrubias AJ, et al. NAD+ metabolism and its roles in cellular processes during ageing. Nat Rev Mol Cell Biol. 2021. PMID 33353981 →
- Rajman L, Chwalek K, Sinclair DA. Therapeutic Potential of NAD-Boosting Molecules: The In Vivo Evidence. Cell Metab. 2018. PMID 29514064 →
Research Use Only
Sold strictly for in-vitro laboratory and scientific research. Not for human or animal consumption. Not a drug, supplement, or medical device. These statements have not been evaluated by the FDA.